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e coli extract with sfii  (New England Biolabs)


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    New England Biolabs e coli extract with sfii
    (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into <t>E.</t> <t>coli</t> and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.
    E Coli Extract With Sfii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+extract+with+sfii/SfiI/bio_rxiv__64898__2026__03__06__710085-216-12-17
    Average 96 stars, based on 926 article reviews
    e coli extract with sfii - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms"

    Article Title: Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms

    Journal: bioRxiv

    doi: 10.64898/2026.03.06.710085

    (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into E. coli and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.
    Figure Legend Snippet: (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into E. coli and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.

    Techniques Used: Transformation Assay, Derivative Assay, Clone Assay, Plasmid Preparation, Purification, Microscopy, Infection, Transfection, Isolation, Sequencing



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    e coli extract with sfii  (New England Biolabs)
    96
    New England Biolabs e coli extract with sfii
    (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into <t>E.</t> <t>coli</t> and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.
    E Coli Extract With Sfii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+extract+with+sfii/SfiI/bio_rxiv__64898__2026__03__06__710085-216-12-17
    Average 96 stars, based on 1 article reviews
    e coli extract with sfii - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into E. coli and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.

    Journal: bioRxiv

    Article Title: Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms

    doi: 10.64898/2026.03.06.710085

    Figure Lengend Snippet: (A) PCR analysis of the three VACV genomic fragments transformed into yeast, performed on pre-selected TAR-derived clones. (B) Restriction enzyme analysis of plasmid DNA from clones C1 and C2 following transformation into E. coli and plasmid purification. (C) Brightfield microscopy images of plaque assays on A549 cells infected with MVA, VACV, the rescue material from cells transfected with pYCC3-VACV_C1 (clone C1), the rescue material from cells transfected with pYCC3-VACV_C2 (clone C2), as well as rescue samples from MVA-infected cells transfected with pYCC3-VACV_C1 (MVA + pYCC3-VACV_C1) or pYCC3-VACV_C2 (MVA + pYCC3-VACV_C2). A549 cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification using brightfield microscopy. (D) Distribution of parental viral genomes within isolated clones obtained from rescue experiments in MVA-infected cells transfected with pYCC3-VACV_C1 or pYCC3-VACV_C2, based on deep sequencing analysis.

    Article Snippet: Restriction profiling of pYCC3-VACV plasmids was performed by digesting 500 ng of E. coli extract with SfiI (New England Biolabs, USA), followed by migration on a 0.8% agarose gel at 120 V for 1 h. Restriction enzymes used in this study included EcoRI, AfeI, PacI, SphI and SfiI (New England Biolabs, USA) as well as SgrDI, MreI and FspAI (Thermo Fisher Scientific, USA).

    Techniques: Transformation Assay, Derivative Assay, Clone Assay, Plasmid Preparation, Purification, Microscopy, Infection, Transfection, Isolation, Sequencing